ABSTRACT: In this presentation, we focus on the analysis of tongue protrusion (TP) as a non-manual negation strategy found in Tiros Sign Language (TSL), a small sign language used in a micro community of 21 individuals (10 deaf and 11 hearing) in the countryside of the state of Minas Gerais (MG), Brazil. We observed that TP was used fairly frequently in Tiros SL, including uses as a non-manual particle signaling negation. As TP has also been observed in other sign languages of the world (e.g., Kata Kolok [Lutzenberger, Pfau & De Vos, 2022], Hong Kong Sign Language [Gan, 2019], Turkish Sign Language [Makaroğlu, 2021], and British Sign Language [Levin & Schembri, 2013]), although not always expressing negation, we aim at providing new data of this feature from a newly described sign language of Brazil.